Differences

This shows you the differences between two versions of the page.

Link to this comparison view

tutorial:headmodel_meg [2012/08/08 16:51]
robert [Procedure]
tutorial:headmodel_meg [2014/11/12 15:03] (current)
jennifer [Reading in the anatomical data]
Line 1: Line 1:
-{{tag>tutorial timelock source meg headmodel mri plot}}+{{tag>tutorial timelock source meg headmodel mri plot MEG-language}}
 \\ \\
  
Line 8: Line 8:
 In this tutorial you can find information about how to construct a volume conduction model of the head (head model) based on a single subject's MRI. We will use the anatomical images that belong to the same subject whose data were analyzed in the preprocessing and averaging tutorials ([[tutorial:preprocessing|Trigger-based trial selection]], [[tutorial:eventrelatedaveraging|Event related averaging and planar gradient]]). The corresponding anatomical MRI data is available from the [[ftp://ftp.fcdonders.nl/pub/fieldtrip/tutorial/Subject01.zip|ftp server]].  In this tutorial you can find information about how to construct a volume conduction model of the head (head model) based on a single subject's MRI. We will use the anatomical images that belong to the same subject whose data were analyzed in the preprocessing and averaging tutorials ([[tutorial:preprocessing|Trigger-based trial selection]], [[tutorial:eventrelatedaveraging|Event related averaging and planar gradient]]). The corresponding anatomical MRI data is available from the [[ftp://ftp.fcdonders.nl/pub/fieldtrip/tutorial/Subject01.zip|ftp server]]. 
  
-The volume conduction model of the head that will be constructed here is specific to the computation and source reconstruction of MEG data. This tutorial will **not** show how to perform the source reconstruction itselfIf you are interested in source reconstruction methods, you can go to the [[tutorial:beamformer|Localizing oscillatory sources using beamformer techniques]] and to the [[tutorial:minimumnormestimate|Source reconstruction of event-related fields using minimum-norm estimate]] tutorials+The volume conduction model of the head that will be constructed here is specific to the computation and source reconstruction of MEG data. Different strategies can be used for the construction of head modelsThe processing pipeline of the tutorial is an example which we think is the most appropriate for the tutorial-dataset
  
-Furthermore, the volume conduction model is MEG specific and cannot be used for source reconstruction of EEG data. If you are interested in EEG source reconstruction methods, you can go to the corresponding [[tutorial:headmodel_eeg|EEG tutorial]].+This tutorial will **not** show how to perform the source reconstruction itself. If you are interested in source reconstruction methodsyou can go to the [[tutorial:beamformer|Localizing oscillatory sources using beamformer techniques]] and to the [[tutorial:minimumnormestimate|Source reconstruction of event-related fields using minimum-norm estimate]] tutorials.  
 + 
 +The volume conduction model created here is MEG specific and cannot be used for EEG source reconstruction. If you are interested in EEG source reconstruction methods, you can go to the corresponding [[tutorial:headmodel_eeg|EEG tutorial]].
  
 ===== Background ===== ===== Background =====
  
-The EEG/MEG signals measured on the scalp do not directly reflect the location of the activated neurons. To reconstruct the location and the time-course or spectral content of a source in the brain, various source-localization methods are available. You can read more about the different methods in review papers suggested [[:references_to_implemented_methods#references_to_review_papers|here]].    +{{page>:tutorial:shared:sourcelocalization_background}}
- +
-The level of the activity at a source location is estimated from  +
-  - the EEG/MEG activity measured on (around) the scalp  +
-  - the spatial arrangement of the electrodes/sensors (**channel positions**),  +
-  - the geometrical and electrical/magnetic properties of the head (**head model**) +
-  - the location of the source (**source model**) +
- +
-Using this information, source estimation comprises two major steps: (1) Estimation of the potential or field distribution for a known source and for a known model of the head is referred to as **forward modeling**. (2) Estimation of the unknown sources corresponding to the measured EEG or MEG is referred to as **inverse modeling**.  +
- +
-The forward solution can be computed when the head model, the channel positions and the source is given. For distributed source models and for scanning approaches (such as beamforming), the source model is discretizing the brain volume into a volumetric or surface grid. When the forward solution is computed, the **lead field matrix** (= channels X source points matrix) is calculated for each grid point taking into account the head model and the channel positions.  +
- +
-A prerequisite of forward modeling is that the geometrical description of all elements (channel positions, head model and source model) is registered in the same coordination system with the same units. As such the exact definition of the coordinate system is irrelevant, but  all geometric information that is needed to obtain a forward model needs to be expressed in the same one. The MEG sensors by default are defined relative to anatomical landmarks of the head (the fiducial coils), therefore when the anatomical images are also aligned to these landmarks, the MEG sensors do not need to be re-aligned. EEG data is typically not aligned to the head, therefore, the electrodes have to  be re-aligned prior to source-reconstruction. You can read [[faq:how_to_coregister_an_anatomical_mri_with_the_gradiometer_or_electrode_positions|here]] and [[example:align_eeg_electrode_positions_to_bem_headmodel|here]] about the re-alignment of the anatomical images, the MEG sensors and EEG electrodes .  +
- +
-{{:tutorial:minimumnormestimate:source_analysis-03.png?500|Source reconstruction}} +
-\\  +
-//Figure 1. Major steps in source reconstruction// +
  
-This tutorial is focusing on how to build the **volume conduction model for the head**. In FieldTrip this is represented as a MATLAB structure which is usually indicated with the variable name **vol**. The  volume conduction model describes how the currents flow through the tissue, not where they originate from. In general it consists of a description of the geometry of the head, a description of the conductivity of the tissue, and mathematical parameters that are derived from these. Whether and how the mathematical parameters are described depends on the computational solution to the forward problem either by numerical approximations, such as the boundary element and finite element method (BEM and FEM), or by exact analytical solutions (e.g. for spherical models).+This tutorial is focusing on how to build the **volume conduction model for the head**. 
  
-The more accurate the description of the geometry of the head or the source, the better the quality of the forward model. There are many types of head models which, to various degrees, take the individual anatomy into account. The different head models available in FieldTrip are listed [[faq:what_kind_of_volume_conduction_models_are_implemented|here]]. In this specific tutorial we will use a semi-realistic head model developed by Nolte (2003) that assumes a realistic information about the interface between the brain and the skull. This outer brain surface will be extracted from the anatomical MRI images of the subject. +{{page>:tutorial:shared:headmodel_background}}
  
-First, we will use anatomical MRI of the subject to extract the brain surface from the anatomical images, which is termed **segmentation**. Note that the segmentation procedure is quite time consuming. Following the segmentation of the anatomical images, a description of the surface using vertices and triangles is constructed. Finally, the single-shell head model will be computed.+In this specific tutorial we will use a semi-realistic head model developed by Nolte (2003) that assumes a realistic information about the interface between the brain and the skull. This outer brain surface will be extracted from the anatomical MRI images of the subject. First, we will use anatomical MRI of the subject to extract the brain surface from the anatomical images, which is termed **segmentation**. Note that the segmentation procedure is quite time consuming. Following the segmentation of the anatomical images, a description of the surface using vertices and triangles is constructed. Finally, the single-shell head model will be computed.
  
 <note> <note>
Line 48: Line 33:
 ===== Procedure ===== ===== Procedure =====
 We will create a head model based on the anatomical mri of the [[tutorial:shared:dataset|tutorial data set]] which is available [[ftp://ftp.fcdonders.nl/pub/fieldtrip/tutorial/Subject01.zip|here]]. The pipeline is depicted in Figure 2. We will create a head model based on the anatomical mri of the [[tutorial:shared:dataset|tutorial data set]] which is available [[ftp://ftp.fcdonders.nl/pub/fieldtrip/tutorial/Subject01.zip|here]]. The pipeline is depicted in Figure 2.
-\\ 
-\\ 
-{{:tutorial:minimumnormestimate:headmodel-01.png?200|Head model pipeline}} 
-\\ 
-//Figure 2. Pipeline of creating a head model// 
 \\ \\
   * First, we will read the anatomical data with **[[reference:ft_read_mri|ft_read_mri]]**;   * First, we will read the anatomical data with **[[reference:ft_read_mri|ft_read_mri]]**;
Line 58: Line 38:
   * and create the headmodel with **[[reference:ft_prepare_headmodel|ft_prepare_headmodel]]**.   * and create the headmodel with **[[reference:ft_prepare_headmodel|ft_prepare_headmodel]]**.
   * Finally, we will check the geometry of the head model by plotting it with **[[reference:ft_plot_vol|ft_plot_vol]]**.   * Finally, we will check the geometry of the head model by plotting it with **[[reference:ft_plot_vol|ft_plot_vol]]**.
 +
 +{{:tutorial:headmodel:headmodel-01.png?250|Head model pipeline}}
 \\ \\
 +//Figure 2. Pipeline of creating a head model//
 +
  
 ===== Reading in the anatomical  data ===== ===== Reading in the anatomical  data =====
  
-Before starting with to use FieldTrip, it is important that you set up your matlab path properly. You can read about how to set up your matlab path [[faq:should_i_add_fieldtrip_with_all_subdirectories_to_my_matlab_path|here]]. +Before starting to use FieldTrip, it is important that you set up your MATLAB path properly. You can read about how to set up your MATLAB path [[faq:should_i_add_fieldtrip_with_all_subdirectories_to_my_matlab_path|here]]. 
  
 <code> <code>
-cd <cd to directory with ft_defaults>; +cd PATH_TO_FIELDTRIP 
-ft_defaults;+ft_defaults
 </code> </code>
 \\ \\
Line 72: Line 56:
 <code> <code>
 mri = ft_read_mri('Subject01.mri'); mri = ft_read_mri('Subject01.mri');
 +
 +disp(mri)
 +          dim: [256 256 256]
 +      anatomy: [256x256x256 int16]
 +          hdr: [1x1 struct]
 +    transform: [4x4 double]
 +         unit: 'mm'
 +     coordsys: 'ctf'
 </code> </code>
 \\ \\
Line 81: Line 73:
   * **coordsys**: The description of the coordinate system which the anatomical data is aligned to.   * **coordsys**: The description of the coordinate system which the anatomical data is aligned to.
  
-You can see that the **coordsys** field of anatomical data that we read in is already aligned to the ctf coordinate system (denoted by 'ctf')+You can see that the **coordsys** field of anatomical data that we read in is already aligned to the [[faq:how_are_the_different_head_and_mri_coordinate_systems_defined#details_of_the_ctf_coordinate_system|ctf coordinate system]]
 \\ \\
 \\  \\ 
-It is also possible to read in other anatomical data-formats with FieldTrip which are defined in a different coordinate system. See this [[:dataformat|this page]] for more information on the data-formats. When you read in your own anatomical data, it may does not give information on the coordinate system of your volume and/or maybe there is no transformation matrix specified. In this case, you can check the coordinate-system with the **[[reference:ft_checkcoordsys|ft_checkcoordsys]]** function. If the anatomical mri is not aligned to the ctf coordinate system, it can be aligned with using the **[[reference:ft_volumerealign|ft_volumerealign]]** +<note> 
- function. For this alignment, you will need to align your mri to the fiducial points (LPA, RPA and nasion). This function does not change the anatomical data, instead it creates the transformation matrix (in the **transform** field) that aligns the anatomical data to the intended coordinate system (in this case CTF, see also [[faq:how_are_the_different_head_and_mri_coordinate_systems_defined|this faq]]).+It is also possible to read in anatomical MRI data in [[:dataformat|other formats]], which are defined in [[faq:how_are_the_different_head_and_mri_coordinate_systems_defined|a different coordinate system]]. If your anatomical MRI is not aligned to the ctf coordinate system, it can be [[faq:how_to_coregister_an_anatomical_mri_with_the_gradiometer_or_electrode_positions|aligned]] using **[[reference:ft_volumerealign|ft_volumerealign]]** 
 + function. For this, you will need to align your MRI to the [[faq:how_are_the_lpa_and_rpa_points_defined|fiducial points]]. 
 \\ \\
 +\\
 +When you read in your own anatomical data, it may not give information on the coordinate system in which the anatomical data is expressed and/or maybe there is no [[faq:how_to_coregister_an_anatomical_mri_with_the_gradiometer_or_electrode_positions|transformation matrix]] specified. In this case, you can check the coordinate-system with the **[[reference:ft_determine_coordsys|ft_determine_coordsys]]** function.
 +</note> 
 +\\
 +
 ===== Segmentation ===== ===== Segmentation =====
  
-In this step, the anatomy of the mri variable is segmented. As the result of the segmentation the gray, white and the cerebro-spinal fluid (csf) compartments in the anatomical data are differentiated. Based on these compartments a brainmask is created. The brainmask is a binary mask of the inner skull (i.e. all voxels which belong to the inner skull or to inside the skull are represented by 1, all other voxels by 0) The function **[[reference:ft_volumesegment]]** will produce the required output.+In this step, the voxels of the anatomical MRI are segmented (i.e. separated) into [[faq:how_is_the_segmentation_defined|different tissue types]] . By default the gray matter, white matter and the cerebro-spinal fluid (csf) compartments differentiated. Based on these compartments a so called brainmask is created, which is a binary mask of the content inside the skull. All voxels that are inside the skull (i.e. the complete brain) are represented by 1, all other voxels by 0. The function **[[reference:ft_volumesegment]]** will produce the required output. 
 <note important> <note important>
-Note that the segmentation is quite time consuming. If you have access to the preprocessed file you can skip ahead to the next step. You can load the segmented MRI (of this tutorial data) available from the [[ftp://ftp.fcdonders.nl/pub/fieldtrip/tutorial/beamformer/segmentedmri.mat|FieldTrip ftp server]] (segmentedmri.mat).+Note that the segmentation is quite time consuming and if you want you can load the result and skip ahead to the next step. You can download the segmented MRI of this tutorial data from the [[ftp://ftp.fcdonders.nl/pub/fieldtrip/tutorial/headmodel_meg/segmentedmri.mat|ftp server]] (segmentedmri.mat).
 </note> </note>
  
 <code> <code>
-% segmentation of the mri 
-load mri; 
 cfg           = []; cfg           = [];
-cfg.coordsys  = 'ctf'; 
 cfg.output    = 'brain'; cfg.output    = 'brain';
-seg           = ft_volumesegment(cfg, mri);+segmentedmri  = ft_volumesegment(cfg, mri);
  
-save seg seg;+save segmentedmri segmentedmri 
 + 
 +disp(segmentedmri) 
 +        dim: [256 256 256] 
 +    transform: [4x4 double] 
 +     coordsys: 'ctf' 
 +         unit: 'mm' 
 +        brain: [256x256x256 logical] 
 +          cfg: [1x1 struct] 
 +         
 </code> </code>
 \\ \\
-The seg variable contains the following fields:+The segmentedmri data structure contains the following fields:
   * **dim**   * **dim**
   * **transform**   * **transform**
Line 111: Line 116:
   * **unit**: unit of measurement of the voxels   * **unit**: unit of measurement of the voxels
   * **brain**: binary brainmask   * **brain**: binary brainmask
-  * **cfg**: configuration information of the function which created seg+  * **cfg**: configuration information of the function which created segmentedmri
  
-The segmentation does not change the coordinate system or the size of the volume. You can see this in the first three fields (dim, transform and coordsys) which are the same as the corresponding fields of the inputmri variable. But now, the field **transform** aligns the matrix in field **brain** (which contains the brainmask) to the coordinate system defined in the **coordsys** field. +The segmentation does not change the coordinate system, nor the size of the volume. You can see this in the first three fields (dim, transform and coordsys) which are the same as the corresponding fields of the input mri data structure. But now, the field **transform** aligns the matrix in field **brain** (which contains the brainmask) to the coordinate system defined in the **coordsys** field. 
 \\ \\
 Alternatively, you can also leave out the definition of the cfg.output. In this case, the function will output the default segmentation that are the probabilistic values of the gray, white and csf compartments. In this case, the brain mask will be automatically created in the next step by the ft_prepare_headmodel function. For further information on the different segmentation options, read the help of **[[reference:ft_volumesegment|ft_volumesegment]]**. Alternatively, you can also leave out the definition of the cfg.output. In this case, the function will output the default segmentation that are the probabilistic values of the gray, white and csf compartments. In this case, the brain mask will be automatically created in the next step by the ft_prepare_headmodel function. For further information on the different segmentation options, read the help of **[[reference:ft_volumesegment|ft_volumesegment]]**.
  
 ===== Head model ===== ===== Head model =====
-Here, we create the volume conduction model of the head from the segmented volume. We will specify method 'singleshell' to build the head model in the cfg.method field. + 
 +Once the brain mask is segmented out of the anatomical MRI, a surface description of the brain is constructed and the volume conduction model . We will specify method 'singleshell' to build the head model in the cfg.method field using **[[reference:ft_prepare_headmodel|ft_prepare_headmodel]]**.
  
 <code> <code>
-load seg; 
  
 cfg = []; cfg = [];
 cfg.method='singleshell'; cfg.method='singleshell';
-vol = ft_prepare_headmodel(cfg,seg);+vol = ft_prepare_headmodel(cfg, segmentedmri);
  
-save vol vol;+save vol vol 
 + 
 +disp(vol) 
 +     bnd: [1x1 struct] 
 +    type: 'singleshell' 
 +    unit: 'mm' 
 +     cfg: [1x1 struct]
 </code> </code>
 +
 +The vol data structure contains the following fields:
 +\\
 +  * **bnd**: contains the geometrical description of the head model.
 +  * **type**: describes the method that was used to create the headmodel.
 +  * **unit**: the unit of measurement of the geometrical data in the bnd field
 +  * **cfg**: configuration of the function that was used to create vol
 +
 +The **bnd** field describes a surface with vertices and triangles (in the **bnd.pnt** and **bnd.tri** fields) as the geometrical description of the volume conductor. 
 +\\
 +<note>This tutorial does not intend to make a elaborative comparison of the different volume conduction models, nor to discuss their relative merits. 
 +
 +The method used in this tutorial is based on [[http://www.ncbi.nlm.nih.gov/pubmed/14680264|Nolte G. (2003) The magnetic lead field theorem in the quasi-static approximation and its use for magnetoencephalography forward calculation in realistic volume conductors]]. We recommend this method for most general situations.
 +
 +The paper [[http://www.ncbi.nlm.nih.gov/pubmed/21828900|Lalancette M, Quraan M, Cheyne D. (2011) Evaluation of multiple-sphere head models for MEG source localization]] discusses another popular method for MEG forward modeling, which is based on fitting local spheres to the surface. This alternative method can be used by specifying cfg.method='localspheres' in **[[:reference:ft_prepare_headmodel]]**.
 +
 +</note> 
 +
  
  
 ===== Visualization ===== ===== Visualization =====
-The head model (vol) contains the geometrical description of the head. You can visualize this using the following code. First, we will plot the sensors with the **[[reference:ft_plot_sens|ft_plot_sens]]** function. Second, we will plot the head model with **[[reference:ft_plot_vol|ft_plot_vol]]** in the same figure with the sensors. In order to plot also the location of the MEG channels, we will read in the location of the channels using the .ds file from the tutorial data and the **[[reference:ft_read_sens|ft_read_sens]]** function. (The .zip file that can be downloaded from the [[ftp://ftp.fcdonders.nl/pub/fieldtrip/tutorial/Subject01.zip|FieldTrip ftp server]] also contains the .ds file.)+The head model (vol) contains the brain-skull boundary as the geometrical description of the head. You can visualize this using the following code. First, we will plot the sensors (MEG channels) with the **[[reference:ft_plot_sens|ft_plot_sens]]** function. Second, we will plot the head model with **[[reference:ft_plot_vol|ft_plot_vol]]** in the same figure with the sensors. In order to plot also the location of the MEG channels, we read in the location of the channels using the .ds file from the tutorial data and the **[[reference:ft_read_sens|ft_read_sens]]** function. (The .zip file that can be downloaded from the [[ftp://ftp.fcdonders.nl/pub/fieldtrip/tutorial/Subject01.zip|FieldTrip ftp server]] also contains the .ds file.) The units of the headmodel are defined in 'cm', while the units of the sensors are in 'mm'. When we plot the headmodel together with the sensors, they need to have the same units. Therefore, the units of the headmodel will be converted to 'cm' with the **[[reference:ft_convert_units|ft_convert_units]]** function.
  
 \\ \\
 <code> <code>
 +vol = ft_convert_units(vol,'cm');
 sens = ft_read_sens('Subject01.ds'); sens = ft_read_sens('Subject01.ds');
  
 +figure
 ft_plot_sens(sens, 'style', '*b'); ft_plot_sens(sens, 'style', '*b');
-hold on+ 
-load vol;+hold on
 ft_plot_vol(vol); ft_plot_vol(vol);
 </code> </code>
Line 148: Line 179:
 //Figure 3. The geometry of the volume conduction  model of the head using method "singleshell"// //Figure 3. The geometry of the volume conduction  model of the head using method "singleshell"//
 \\ \\
-When the figure is plotted, you can look at the figure from different views using the curved arrow in the MATLAB figure menu.+When the figure is plotted, you can look at the figure from different views using the curved arrow in the MATLAB figure menu. Note that there are 4 channels hovering above the normal channels; those are the MEG reference channels that can be used for environmental noise suppression
 \\ \\
-==== Exercise 1 ====+===== Exercise 1 =====
  
 <note exercise> <note exercise>
-   * Create a head model with method 'singlesphere'  that you fit on the inside brain surface (i.e. using the output of the already made segmentation)Visualize it! Plot both head models in the same figure. (Check the help of **[[reference:ft_plot_vol|ft_plot_vol]]** for further options of the visualization (e.g. color, transparency) that can help to see the two head models together.What is the difference between the head models? +   * Create a head model with method 'singlesphere' that you fit on the inside brain surfacei.e. using the output of the already made segmentation. 
 +   * Plot both head models in the same figure, check the help of **[[reference:ft_plot_vol|ft_plot_vol]]** for further options of the visualization (e.g. color, transparency) which help to see the two head models together. 
 +   * What is the difference between the head models? 
 </note> </note>
 \\ \\
-===== The vol structure ===== 
  
-We used the name vol for the matlab variable that contains the head model. The vol contains the following fields: +===== Exercise 2 =====
-\\ +
-  * **bnd**: This field contains the geometrical description of the head model. +
-  * **type**: This field described the method that was used to create the headmodel. +
-  * **unit**: the unit of measurement of the geometrical data in the bnd field +
-  * **cfg**: configuration of the function that was used to create vol +
- +
-You can see 'nolte' in the **type** field. This refers to the method 'singleshell' which was used to create vol. The **bnd** field defines points and triangulated surfaces (in the **bnd.pnt** and **bnd.tri** fields) as the geometrical description of the head model. Note that the unit of measurement used in this geometrical description is in 'cm'. This is different from the units of the segmented volume (seg). This conversion of units happened because the sensors are also defined in 'cm', and so the units of all type of geometrical information will be the same when later the leadfield will be computed. (You can see the units of the sensors in the earlier created matlab variable, sens, in its unit field.) +
-\\ +
-==== Exercise 2 ====+
  
 <note exercise> <note exercise>
Line 175: Line 198:
  
 ===== Summary and further reading ===== ===== Summary and further reading =====
-In this tutorial, it was explained how to build a volume conduction model of the head using a single subject anatomical mri and the single shell method developed by Nolte (2003). We also compared the head model to a single sphere that was fitted on the inside brain surface. +In this tutorial, it was explained how to build a volume conduction model of the head using a single subject anatomical mri and the single shell method developed by Nolte (2003). In the exercises, we compared the head model to a single sphere that was fitted on the inside brain surface. 
  
 You can read more about specific source-reconstruction methods in the [[tutorial:beamformer|Localizing oscillatory sources using beamformer techniques]] and in the [[tutorial:minimumnormestimate|Source reconstruction of event-related fields using minimum-norm estimate]] tutorials. You can read more about specific source-reconstruction methods in the [[tutorial:beamformer|Localizing oscillatory sources using beamformer techniques]] and in the [[tutorial:minimumnormestimate|Source reconstruction of event-related fields using minimum-norm estimate]] tutorials.
Line 181: Line 204:
 Here are the related [[:faq|faqs]]: Here are the related [[:faq|faqs]]:
 \\ \\
-{{topic>faq +headmodel +meg &list}} +{{topic>headmodel meg +faq &list}}
-\\+
 and the related [[:example|example scripts]]: and the related [[:example|example scripts]]:
 \\ \\
-{{topic>example +headmodel +meg &list}} +{{topic>headmodel meg +example&list}}
-\\+
  
tutorial/headmodel_meg.1344437472.txt.gz · Last modified: 2012/08/08 16:51 by robert

You are here: starttutorialheadmodel_meg
This DokuWiki features an Anymorphic Webdesign theme, customised by Eelke Spaak and Stephen Whitmarsh.
www.chimeric.de Valid CSS Driven by DokuWiki do yourself a favour and use a real browser - get firefox!! Recent changes RSS feed Valid XHTML 1.0